About Redox probe calibration #
In order for your Redox probe to remain accurate, it must be calibrated on a regular basis. New Redox probes must always be calibrated before being put to use.
Probe maintenance and care #
In order for the ProfiLux to give you the most accurate probe readings, proper calibration must be performed. Here are some general tips to go by:
- Calibrate NEW probes before being put to use; pH, redox, conductivity
- If you are going to calibrate a probe that is already in-use, make sure to first clean it with warm water or 50/50 mix of water and white vinegar to remove build-up
- In-between calibration steps, rinse and wipe the probe to prevent any cross-contamination between fluids
- ALWAYS make sure the probe tip is kept clean and free from any detritus or foreign material
- Make sure NO microbubbles get caught on the tip of the probe
- Calibrate every 1 – 3 months
Probe calibration terminology #
Before calibrating your probe, we recommend you become familiar with the terminology used during the calibration process.
Calibration tolerance
Used for telling the ProfiLux how much of a measurement swing is allowed during calibration. The larger the number, the large the tolerated swing, at the cost of probe accuracy.
By default, and in most cases, a calibration tolerance of 2 can be used.
A tolerance of 2 or 3 can be used for probes less than a year old. Probes greater than one year old, can use a tolerance of 4 or 5.
Calibration value 2
The mV value of the calibration fluid that will be used
What you’ll need… #
In order to proceed with Redox probe calibration, you will need the following:
| 220mV calibration fluid (PL-0072) |
| Null plug (PL-1212) |
Null plug is required for calibration
The Redox probe calibration process requires you to use a special plug called, Null plug. This is the blank BNC connector plug that was included with the ProfiLux 4 / 4e product box and Redox expansion cards. Please have this plug on-hand because you will need it for the calibration steps.
This Null plug will connect to the Redox port on the ProfiLux.
Listen for the 3 beeps!
During calibration, the ProfiLux will give the probe 900s (15 minutes) for the ADC values to stabilize. For steps 7 and 8, when you hear 3 beeps, the calibration step is complete. The amount of time it will take to hear these beeps will depend on probe age and set Calibration Tolerance.
Redox probe calibration steps #
- Press any of the arrow keys on the ProfiLux and select Sensor settings
- Select Redox 1 or desired Redox probe you want to calibrate
- Select Calibration
- Select (1) for Cali.tolerance if probe is brand new or less than one year old
5. Select 220mV for Calibration value 2
6. Disconnect the Redox probe from the ProfiLux and connect the Null plug to the Redox port. Press the (√) key to continue.
7. The ProfiLux will now begin the countdown. Leave the null plug connected. Wait for the 3 beeps before proceeding to the next step.
8. Disconnect the null plug, reconnect the Redox probe, then dip the probe in the 220mV fluid and press the (√) key. When you hear the 3 beeps, select YES to save calibration.
9. Wipe the probe and put it back into the aquarium
